LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1538..1634) /label=attL2 /note="attL2 (c)" misc_signal complement(1649..1668) /note="T7 Promoter/priming site (c)" primer_bind 1676..1692 /note="M13 Reverse priming site" gene 1805..2614 /note="Kanamycin resistance gene" rep_origin 2735..3408 /note="pUC origin" vector join(1546..3411,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1531 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26282 IMAGE:4824054" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..1531 /gene="GNPTG" /gene_synonym=LP2537 /db_xref="GeneID:84572" /db_xref="HGNC:23026" /db_xref="MIM:607838" CDS 692..1531 /dnas_title="N-acetylglucosamine-1-phosphate transferase, gamma subunit" /gene="GNPTG" /gene_synonym=LP2537 /codon_start=1 /product="N-acetylglucosamine-1-phosphate transferase, gamma subunit" /protein_id="AAH14592.1" /db_xref="GI:15779035" /db_xref="GeneID:84572" /db_xref="HGNC:23026" /db_xref="MIM:607838" /translation="MAAGLARLLLLLGLSAGGPAPAGAAKMKVVEEPNAFGVNNPFLP QASRLQAKRDPSPVSGPVHLFRLSGKCFSLVESTYKYEFCPFHNVTQHEQTFRWNAYS GILGIWHEWEIANNTFTGMWMRDGDACRSRSRQSKVELACGKSNRLAHVSEPSTCVYA LTFETPLVCHPHALLVYPTLPEALQRQWDQVEQDLADELITPQGHEKLLRTLFEDAGY LKTPENEPTQLEGGPDSLGFETLENCRKAHKELSKEIKRLKGLLTQHGIPYTRPTETS NLEHLGHETPRAKSPEQLRGDPGLRGSL" misc_feature 692..1531 /note="GNPTG coding region" misc_difference 1288..1289 /gene="GNPTG" /gene_synonym=LP2537 /note="3 bases in the human genome, GAA, are not found in cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgcgaagatg aaggtggtgg aggagcccaa 721 cgcgtttggg gtgaacaacc cgttcttgcc tcaggccagt cgcctccagg ccaagaggga 781 tccttcaccc gtgtctggac ccgtgcatct cttccgactc tcgggcaagt gcttcagcct 841 ggtggagtcc acgtacaagt atgagttctg cccgttccac aacgtgaccc agcacgagca 901 gaccttccgc tggaacgcct acagtgggat cctcggcatc tggcacgagt gggagatcgc 961 caacaacacc ttcacgggca tgtggatgag ggacggtgac gcctgccgtt cccggagccg 1021 gcagagcaag gtggagctgg cgtgtggaaa aagcaaccgg ctggcccatg tgtccgagcc 1081 gagcacctgc gtctacgcgc tgacgttcga gacccccctc gtctgccacc cccacgcctt 1141 gctagtgtac ccaaccctgc cagaggccct gcagcggcag tgggaccagg tagagcagga 1201 cctggccgat gagctgatca ccccccaggg ccatgagaag ttgctgagga cactttttga 1261 ggatgctggc tacttaaaga ccccagaaaa tgaacccacc cagctggagg gaggtcctga 1321 cagcttgggg tttgagaccc tggaaaactg caggaaggct cataaagaac tctcaaagga 1381 gatcaaaagg ctgaaaggtt tgctcaccca gcacggcatc ccctacacga ggcccacaga 1441 aacttccaac ttggagcact tgggccacga gacgcccaga gccaagtctc cagagcagct 1501 gcggggtgac ccaggactgc gtgggagttt gTAGGACCCA GCTTTCTTGT ACAAAGTTGG 1561 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 1621 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 1681 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 1741 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 1801 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 1861 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 1921 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 1981 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 2041 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 2101 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 2161 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 2221 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 2281 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 2341 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 2401 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 2461 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 2521 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 2581 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 2641 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 2701 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 2761 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 2821 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 2881 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 2941 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3001 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3061 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3121 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 3181 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 3241 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 3301 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 3361 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //